Pclbw cox8-egfp-mcherry
SpletpCLBW cox8 EGFP mCherry Plasmid #78520 Purpose Fluorescent mitophagy reporter Depositor David Chan Article Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi: Insert none Use Retroviral Tags cox8 EGFP mCherry Expression Mammalian Mutation Promoter CMV Availability Academic Institutions and Nonprofits only Enlarge pGEX-4T HHARI RBR … Splet红色荧光蛋白变种mCherry是一种目前被广泛用于生物技术作为示踪剂的红色 荧光染料 ,包括分子的标记和细胞组分的定位等。. mCherry因为其颜色和单体分子的 光稳定性 ,细胞毒性低。. 比其它荧光蛋白标签更优异,其最大激发光和发射光分别为587nm和610nm。. 从 ...
Pclbw cox8-egfp-mcherry
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SpletAd-mCherry-GFP-LC3B是碧云天自行研发的重组腺病毒,感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿色荧光蛋白 (GFP)和LC3B的融合蛋白,呈现明亮的红色和绿色荧光,可以用于细胞自噬的检测。 自噬小体在与溶酶体融合过程中,溶酶体内的酸性环境会导致GFP荧光淬灭,这为追踪GFP-LC3B的细胞定位增加了难度。 mCherry是一种来自于蘑菇 …
Splet24. jul. 2024 · pCLBW cox8 EGFP mCherry. catalog : 78520. more info or order : Addgene product webpage. citations: 15. Reference; Li C, Li L, Yang M, Yang J, Zhao C, Han Y, et al. PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy. Splet13. okt. 2024 · a Representative image of celastrol-induced mitophagy in HeLa, Nur77 −/− HeLa, and Nur77 −/− HeLa cells transfected with Myc-Nur77 by EGFP-mCherry-COX8 assay as described in Methods. Scale ...
Splet15. mar. 2024 · pCLBW cox8-EGFP-mCherry plasmid were transiently transfected into MC38 PINK1 overexpressing cells and their parental controls. After 24 h, cells were … SpletProduct Description. pETDuet-1-SopB-EGFP-Ssb-mCherry is available at Gentaur for Next week Delivery. Precision: The pETDuet promotor is combined with a 1 vector sequence and a SopB gene in the plasmid vector map.. Assay procedure summary: Gentaur supplies 2ug of lyophilized ETDuet-1-So Plasmid in powder form at 4°C to be centrifuged at 5000rpm ...
SpletArticle Snippet: The tandem mitochondrial-targeted mCherry-eGFP mitophagy reporter plasmid (pCLBW-cox8-EGFP-mCherry) was a gift from David Chan (Caltech, Pasadena, …
Splet24. jul. 2024 · pCLBW cox8 EGFP mCherry catalog : 78520 more info or order : Addgene product webpage citations: 15 Reference Li C, Li L, Yang M, Yang J, Zhao C, Han Y, et al. … how to download dht.h librarySpletpCLBW cox8 EGFP mCherry Plasmid #78520 Purpose Fluorescent mitophagy reporter Depositor David Chan Article Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi: Insert none Use Retroviral Tags cox8 EGFP mCherry Expression Mammalian Mutation Promoter CMV Availability Academic Institutions and Nonprofits only Enlarge least used station 2022SpletSo, 487 kilograms in pounds = 487 times 2.204622622 = 1073.6512168403538 pounds. See details on the formula below on this page. least used words in english languageSplet05. nov. 2024 · EGFP-LC3 (#11546), ptfLC3 (#21074), and Cox8-EGFP-mCherry (#78520) were purchased from Addgene. Plasmids were transfected with Lipofectamine 3000 according to the manufacturer’s protocol (Thermo Fisher Scientific, #L3000008). After transfection, tubule cells were incubated for 24 hours. The fluorescent signal was … how to download didnapperSpletThe pCLBW cox8 EGFP mCherry promotor is combined with a vector sequence and a gene in the plasmid vector map. Conjugate The Green Fluorescent Protein (eGFP) of pCLBW cox8 mCherry can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. least useful meaningSplet04. jan. 2024 · WT and miR-379KO MMC MMC treated with NC siRNA or Fis1 siRNA were transfected with pCLBW-cox8-EGFP-mCherry. and then treated with HG (25 mM) or NG (5.5 mM) for 5 days at 37 °C and 5% CO. … how to download dialpad appSpletCOX8-EGFP-mCherry-ACTIN co-expressing HeLa cellline was further established based on the COX8-EGFP expressing cells with lentiviral transfection system. From the as-constructed HeLa cells, we could monitor the cell activities in real time using the living cell workstation. As shown inFigure 1C, with the how to download didi app iphone