WebApr 13, 2024 · GPx activity was analyzed according to the method proposed by Paglia and Valentine ( 1967 ). Supernatant (50 µL) was added to 450 µL of buffer reagent solution (pH 7.0; 5 M K 2 HPO 4, 5 M KH 2 PO 4, 3.5 mM reduced glutathione, 1 mM sodium azide, 2 U glutathione reductase, and 0.12 mM NADPH) and 200 µL of 20 mM H 2 O 2. WebGPX activity was determined at 340 nm by a spectrophotometer as described by Paglia and Valentine (1967). This method is based on NADPH oxidation in the presence of H 2 O 2 as substrate . SOD activity was measured as described by Delmas-Beauviex (1995). This method is based on the reduction of nitroblue tetrazolium in a xanthine- oxidase ...
Paglia, D.E. and Valentine, W.N. J. Studies on the …
WebJan 1, 2000 · The principle of the coupled enzymatic assay first described by Paglia and Valentine (1967) was applied. Glutathione (GSH), glutathione reductase (GR) and NADPH dissolved in phosphate buffer and sample were mixed in cuvettes. Then tert -butylhydroperoxide (BHPx) was added to initiate the reaction in a total volume of 0.8 mL … WebStudies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Paglia DE, Valentine WN The Journal of Laboratory and Clinical Medicine , 01 Jul 1967, 70 (1): 158-169 PMID: 6066618 Share this article Abstract No abstract provided. Citations & impact Impact metrics 4,805 Citations Jump to Citations 1 Data citation mn national guard generals
Analysis of chemical composition of polysaccharides
WebPaglia, D.E. and Valentine, W.N. J. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Lab. Clin. Med.( 1967); 70:158-69. … WebD.E. Paglia and W. N. Valentine (1967) J. Lab. Clin. Med. 70: 158 – 169. UV Method CAT. No. R1 Buffer 60 mL R2 NADPH Lypholized 1 vial R3 Substrate 1 mL REAGENTS R2 at -20ºC 50 Tests R1 and R3 at +2 to +8ºC GLUTATHIONE PEROXIDASE GP 2524 Tele: 02-33385184 Mobil: 0109 – 349 20 77 ... WebJul 1, 2005 · Whole blood and tissue GSH-Px activities were determined by the method of Paglia and Valentine (1967) using H 2 O 2 or cumene hydro-peroxide as substrates. Red cell lysate was prepared by mixing 0.10 ml blood with 0.9 ml distilled water kept cool in ice. mn national golf mn