Irf1 binding motif
WebFeb 12, 2016 · Among the computationally identified regulators, siRNA-mediated gene silencing of interferon regulatory factor 1 ( IRF1) in macrophages significantly decreased the expression of IRG1/CAD at the gene and protein level, which correlated with a reduced production of itaconic acid. WebApr 11, 2024 · IFNγ binds a specific cell-surface receptor complex, a heterodimer of IFNGR1 and IFNGR2, expressed in most cell types, which employ JAK1 and JAK2 to phosphorylate STAT1 (pSTAT1). pSTAT1 homodimers translocate to the nucleus and stimulate the expression of a set of genes, including transcription factors such as IFN regulatory factor …
Irf1 binding motif
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WebNon-alcoholic fatty liver disease (NAFLD) is characterized by accelerated lipid deposition, aberrant inflammation, and excessive extracellular matrix production in the liver. Short of effective intervention, NAFLD can progress to cirrhosis and hepatocellular carcinoma. In the present study we investigated the involvement of C-C motif ligand 11 (CCL11) in NAFLD … WebJan 1, 2013 · IRF1 is a transcription factor involved in interferon signaling and has been shown to harbor tumor suppressor activity. In order to comprehensively identify …
WebJun 15, 2015 · Irf1 binding motif was enriched in the promoters of Batf2-regulated genes. Coimmunoprecipitation study demonstrated Batf2 association with Irf1. Furthermore, Irf1 knockdown showed downregulation of IFN-γ- or LPS/HKTB-activated host-protective genes Tnf, Ccl5, Il12b, and Nos2. WebMay 12, 2024 · IRF1 counteracts IRF4-driven Il9 promoter activity, and IRF1 and IRF4 have opposing function on activating histone modifications, thus modulating RNA polymerase II recruitment. IRF1 occupancy...
WebJun 19, 2024 · Deficiencies in both the IRF1 and BATF genes resulted in decreased binding of AhR, and IRF1 was required for the recruitment of c-Maf. ATAC-seq was used to compare neutral cytokine conditions... WebAbstract. Interferon Regulatory Factor-1 (IRF-1) acts as a transcriptional activator in interferon system and as a tumor suppressor. The loss of functional IRF-1 has been …
WebMar 29, 2024 · EGR2, early growth response 2; IRF1, interferon regulatory factor 1; LV, lentiviral vectors for overexpression; si, small interfering RNA; TEAD4, TEA domain family member 4; TMZ, temozolomide. ... activity in mutant or truncated binding site 2 (motif: TATACACACACACACGGAC)-treated U87 cells, indicating that the motif 2 was the binding …
WebNov 1, 2011 · IRF1 is a transcription factor that participates in interferon signaling. Previous studies of IRF1 binding have utilized in vitroassays. We used ChIP-seq in human … sjw injury attorneys las vegasWebApr 3, 2024 · Mechanistically, upregulated ZEB1, by interacting with C-Terminal Binding Protein (CtBP), suppresses the transcription of IRF1 through binding to its promoter. In addition, IFN-γ can promote EMP in human nasal epithelial cells via p38/ERK mitogen activated protein (MAP) kinase signaling [ 146 ] and in prostate cancer via microRNA … sutter health lab lincoln caWebMar 9, 2024 · We generated endothelial cell-specific interferon regulatory factor 1 (Irf1) knockout (Irf1EC-/−) and Irf1fl/flcontrol mice for use in an endotoxemic murine model of acute lung injury (ALI). In vitrostudies (qPCR, immunoblotting, and ChIP-qPCR) were conducted in mouse lung endothelial cells (MLECs) and HLMVECs. sutter health lab lodi caWebNov 15, 2024 · IRF-binding motif, which, when mutated, abolishes the in-duction of Mda5. This sequence is strongly conserved in the ... targeted the IRF1 binding se-quence, while the reverse primer (mutated box ... sutter health lab in auburn caWebIRF1 is a broadly acting antiviral effector and exhibits tumor-suppressor functions in multiple cellular contexts ( 24, 25 ). With respect to PDA, prior studies have shown that IRF1 can promote a differentiated epithelial cell state and inhibit cell proliferation ( 26, 27 ). sjw logistics llcWebNational Center for Biotechnology Information sjw land surveyingWebOct 1, 2024 · Irf1- regulated 90 molecules contribute to the inflammation and viral defense. • Abstract Methods IFN-γ (50 ng/mL) or IL4 (20 ng/mL) was utilized to stimulate bone marrow-derived macrophages from mice for 24 h for M1- and M2-polarized macrophage model construction, respectively. sjw insurance