Callus induction benthamiana
WebJan 21, 2024 · First, the callus induction was performed on MS medium supplemented with 1 mg/L 2,4-D, 0.1 mg/L NAA, 0.2 mg/ L 6-BA, 0.01 mg/L TDZ, 0.1 g/L Tim, 0.1 g/L Cefo, … WebNicotiana benthamiana is a commonly used model plant for studying plant–pathogen interactions because it is host to numerous plant pathogens and because many research tools are available for this species. ... 1 mg/L NAA, and 0.3 mg/L kinetin (1N0.3K) for plant regeneration. Callus formation occurred using protoplasts after 1 month of ...
Callus induction benthamiana
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WebCallus induction and shoots regeneration from callus was obtained by using MS medium modified with 1-naphthalene acetic acid (auxin) and 6-benzylaminopurine (cytokinin). … WebNov 3, 2024 · The callus sample 9 d after induction was fixed in 80% ethanol (EtOH). After exchanging from ethanol to methanol, the fixed callus were treated three times with methanol at 25°C for 30 min, three times with acetone at 25°C for 30 min, three times with methanol/chloroform (1:1, v/v) at 25°C for 30 min, and then dried at 65°C for 18 h.
WebWe established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar 'Shine Muscat' (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a dormant cane. Agrobacterium strain LBA4404 harboring the binary plasmid pBin19-sgfp, … WebOct 28, 2013 · Callus Induction by the Reacquisition of Embryonic or Meristematic Fate. Numerous studies in recent years have shown that ectopic overexpression of embryonic …
WebThe study was aimed to identify the best media combinations for callus induction, somatic embryogenesis and shoot regeneration of elite sugarcane (Saccharum officinarum L.) genotype Co 86032. Furthermore, optimal dose of antibiotic selection agent Geneticin and Agrobacterium mediated transformation protocol has also been standardized. WebOct 1, 2024 · Selected N. benthamiana plants with good growth were infiltrated with the supernatant at the lower epidermis of the leaves with a 1 ml syringe, without the tip, and labeled accordingly. ... Ding, W.J.; Jin, S.H.; Wang, Y. Callus induction and plant regeneration from leaves of peony. Hortic. Environ. Biotechnol. 2024, 59, 575–582. …
WebInduction of a pluripotent cell mass, called callus, from detached organs is an initial step in in vitro plant regeneration, during which phytohormone auxin-induced ectopic activation of a root developmental program has …
We previously demonstrated that the four Arabidopsis LBD transcription factors (LBD16, LBD17, LBD18 and LBD29) act downstream of ARF7 and ARF19 to direct auxin-induced callus formation15. To gain further insight into the molecular control of auxin-induced callus formation, we used LBD17 as a bait and … See more To examine whether AtbZIP59, as a partner of LBDs, is also involved in directing auxin-induced callus formation, we obtained a transfer DNA (T-DNA) insertion mutant of … See more To investigate the tissue-specific expression of AtbZIP59, we generated transgenic Arabidopsis plants harbouring a ProAtbZIP59::GUS transgene. A GUS staining assay … See more Since AtbZIP59 and LBD could form a heterodimer, it is likely that they share a common downstream target to direct callus formation. A … See more To further explore the interactive role of AtbZIP59 and LBD in auxin-induced callus formation, we generated a lbd16-2 air1-2 double mutant and examined its callus-forming capacity. … See more elf wearing furWebNational Center for Biotechnology Information footprints shoes couponsWebWe observed an increase of callus induction (up to 38%) and plant regeneration (up to 50%) at particular concentrations within the picomolar and nanomolar range. On the … elf what\\u0027s your favorite colorhttp://www.sjfsci.com/en/article/doi/10.12172/202411300001 elf whaleWebcallus [kal´us] 1. localized hyperplasia of the horny layer of the epidermis due to pressure or friction. 2. an unorganized network of woven bone formed about the ends of a broken … elf what breakfast does buddy makeWebOct 16, 2024 · Agrobacterium-mediated transformation of Nicotiana benthamiana leaf cells was used to investigate subcellular localization of its coding proteins . The GFP fluorescence was observed at 40× magnification with a confocal laser scanning microscope (A1+, Nikon, Shanghai, China). ... Jeong, B.R. Callus induction and browning suppression in tree ... elf wheelchairWebNov 24, 2024 · N. benthamiana intact leaves were infiltrated with Agrobacterium strains carrying the reporter construct with (right panel) or without (left panel) ... Callus induction from root explants of wild-type (Col), single mutants bp-5 and er-105, and double mutant bp-5 er-105. Fig. S6. elf welk theatre-escondido